fitctf

This program estimates CTF parameters in batch. The results should be checked in CTFIT.

fitctf <input file> [sf=<SF File>] [apix=<a/pix>] [V=<voltage>] [Cs=<spher aber>] [over=<oversampling>] [acmax=<max AC>] [acfix=<max AC>] [debug]

Parameters:


<input file>File containing boxed particles to be evaluated
[sf=<SF File>]File containing estimated 1D structure factor for this specimen
[apix=<a/pix>]Angstroms/pixel in the image
[V=<voltage>]Voltage in KV
[Cs=<spher aber>]Spherical aberation in mm
[over=<oversampling>]eg - 2 -> 2x oversampling
[acmax=<max AC>]eg - .1 is max 10% ac, default is .14
[acfix=<max AC>]fixed amplitude contrast
[debug]Verbose debugging output

Usage:

fitctf jj1023.hed groel.sm

Description

This program will attempt to determine the CTF parameters of a a set of particles. It will write the result into a .tnf file, which can then be read into ctfit. The results of this program should always be manually verified in ctfit. This program is just designed to save the user a little manual effort.

If you have a fairly small model (size in pixels<128) or are very far from focus, you might consider using the oversampling option with a value of 2 or 3.

Please note that this program uses an exceptionally inefficient algorithm. However, there is a lot of embedded 'magic' in this routine. It is designed to be functional, not fast.


EMAN Manual page, generated Fri Feb 8 16:12:24 2002