<input file> | File containing boxed particles to be evaluated |
[sf=<SF File>] | File containing estimated 1D structure factor for this specimen |
[apix=<a/pix>] | Angstroms/pixel in the image |
[V=<voltage>] | Voltage in KV |
[Cs=<spher aber>] | Spherical aberation in mm |
[over=<oversampling>] | eg - 2 -> 2x oversampling |
[acmax=<max AC>] | eg - .1 is max 10% ac, default is .14 |
[acfix=<max AC>] | fixed amplitude contrast |
[debug] | Verbose debugging output |
fitctf jj1023.hed groel.sm
This program will attempt to determine the CTF parameters of a a set of particles. It will write the result into a .tnf file, which can then be read into ctfit. The results of this program should always be manually verified in ctfit. This program is just designed to save the user a little manual effort.
If you have a fairly small model (size in pixels<128) or are very far from focus, you might consider using the oversampling option with a value of 2 or 3.
Please note that this program uses an exceptionally inefficient algorithm. However, there is a lot of embedded 'magic' in this routine. It is designed to be functional, not fast.