<imagefile> | File containing class averages to be used |
[sym=<c?>] | Cn symmetry if any |
[proc=<num proc>] | Number of processors to use |
[rounds=<2-5>] | Rounds of Euler angle determination to use |
[mask=<rad>] | Mask radius |
[lp=<radius>] | Lowpass filter radius in Fourier pixels |
startAny classes.hed sym=c3 mask=42
This program uses cross common lines to determine the relative orientations of a set of class averages, then builds a 3d model from them. This will not work well on individual particle images, as they are generally too noisy. Typically startnrclasses is used to generate reference free class averages. The user then manually selects 10 or 20 of these class-averages, then runs them through startAny to generate a 3D model.
Despite a great deal of time and effort put into this program, it still doesn't work well on some data sets. Usually it will generate results which are adequate for use as a preliminary model for refinement.