classhelix

This will take a set of filament 'particles' and generate a class-average

classhelix <input file> <output file> [ref=<n>,<start file>] [keep=<sigmult>] [hard=<hard thr>] [first=<n>] [last=<n>]

Parameters:


<input file>Images to be processed
<output file>Processed output
[ref=<n>,<start file>]Image to reference alignments to (must be twice the height of <input> images)
[keep=<sigmult>]Sigma threshold for keeping particles
[hard=<hard thr>]A fixed threshold for particle inclusion
[first=<n>]Limit the range of 'particles' to use.
[last=<n>]Limit the range of 'particles' to use.

Usage:

classhelix centered.hed avg.hed keep=1

Description

Warning: This program is still experimental

This generates a class-average of helix particles. This differs from programs like classalign2 in the allowable translations/roations. In this program, only vertical translation is allowed for the alignment. In addition, the final result is twice the length of a single 'particle'.


EMAN Manual page, generated Mon Jun 10 22:20:46 2002