<input file> | Images to be processed |
<output file> | Processed output |
[trans] | Centers the particle horizontally |
[rot] | Rotates the particle so it is as vertical as possible |
[htov] | Rotates horizontal particles 90 degrees |
[unbend] | Try to unbend the particles so they are truely vertical |
[clip=<width>] | Clip the width of the centered images to <width> pixels |
[despot] | Applies a bright-spot filter to the image |
alignhelix start.hed aligned.hed rot trans
WARNING: This program is still experimental
This program will take a set of filament 'particles', and align them so they are horizontally centered and vertical in the box. The particles should span, or almost span, the box vertically. the box need not be square, but it should at least be a good size for FFT's.