<input file> | Images to be processed |
<output file> | Processed output |
[ref=<n>,<start file>] | Image to reference alignments to (must be twice the height of <input> images) |
[keep=<sigmult>] | Sigma threshold for keeping particles |
[hard=<hard thr>] | A fixed threshold for particle inclusion |
[first=<n>] | Limit the range of 'particles' to use. |
[last=<n>] | Limit the range of 'particles' to use. |
classhelix centered.hed avg.hed keep=1
Warning: This program is still experimental
This generates a class-average of helix particles. This differs from programs like classalign2 in the allowable translations/roations. In this program, only vertical translation is allowed for the alignment. In addition, the final result is twice the length of a single 'particle'.