EMAN2 TUTORIALS

Beginners (introductory tutorials)

Please note that we are continuously developing and updating the tutorials. Items that are greyed out are planned but the tutorials have not yet been completed. Most tutorials include the necessary data. If you have a request for a particular tutorial please feel free to email sludtke@bcm.edu.

Single particle analysis (SPA) - 2D particles

• EMAN1 to EMAN2 Transition Guide - Also with useful quickstart guides for users of other software

• Tutorial from the 2011 EMAN2 workshop (Data on same page) - Based on EMAN2.01. Full single particle reconstruction tutorial

• Tilt Validation tutorial from the Oxford 2012 mini-workshop - Requires EMAN 2.05+

• Random Conical Tilt Tutorial - Requires EMAN 2.05+

Obsolete tutorials still provided for data access, etc.

• Single particle reconstruction tutorial from 2008 Workshop ( - Full tutorial on single particle reconstruction using the workflow (uses Workshop demo data DVD)

Single particle tomography (SPT) - 3D particles

• Single particle tomography USER'S GUIDE (updated on June 2012; under major refactoring do to extensive changes in e2spt capabilities; look for an extensively different version soon)

  e2spt_users_guide_06_2012.pdf

• Data needed for the User's Guide up to 06/2012

  e2spt_data.zip

2D assessment of heterogeneity

• Full tutorial on 2-D analysis for assessment of structural heterogeneity and population dynamics (under construction)

Adding an e2program to the program manger workflow

• Modifying the EMAN2 projectmanager workflow

Advanced (EMAN2 through the ''e2.py'' Python prompt)

* Generate and display an EMAN2 test image

* Rotate, translate, scale or mirror an image

* Add and multiply images, add and multiply by constants

* Convert a real/imaginary image into an amplitude/phase image and vice versa

* Iterate through the pixels of an image

* Make a projection of a 3D model, project through the asymmetric unit

* Change log level in EMAN2