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| == EMAN2 == EMAN2 is now stable enough to be a complete replacement for EMAN1 for most tasks. It is capable of single particle reconstructions at near-atomic resolution, and can process very large data sets (>100,000 particles). |
= EMAN2.2 = Most of the pages are editable by any user that has registered an account on the server. To prevent spam, you need to email sludtke@bcm.edu to get an account on the system if you wish to contribute changes. If you just wish to browse, you don't need an account. |
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| '''''Please read this [[EMAN2/DatabaseWarning|Important Warning]]''''' | EMAN2 is the successor to [[EMAN1]]. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. EMAN2 is capable of processing very large data sets (>100,000 particle) very efficiently (up to 20x faster than EMAN1). |
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| * [[EMAN2/Install|Installation Instructions and Tips]] | Please also note that this is '''not''' the (related) [[EMEN2]] electronic notebook, but is EMAN2, a scientific image processing suite. === Please Cite === EMAN is free software, supported by NIH Grants. It is critical that you cite EMAN2 when you use it in a publication in any significant way, to help us document usage when trying to renew our funding. Feel free to cite any of these: * '''Primary EMAN2 paper:''' * G. Tang, L. Peng, P.R. Baldwin, D.S. Mann, W. Jiang, I. Rees & S.J. Ludtke. (2007) EMAN2: an extensible image processing suite for electron microscopy. J Struct Biol. 157, 38-46. PMID: 16859925 * '''EMAN2 high resolution refinement methods:''' * J.M. Bell, M. Chen, P.R. Baldwin & S.J. Ludtke. (2016) High Resolution Single Particle Refinement in EMAN2.1. Methods. 100, 25-34. PMC4848122 * '''Methods for analysis of conformational and compositional variability:''' * Ludtke, S. J. "Single-Particle Refinement and Variability Analysis in EMAN2.1." in Methods Enzymol 579159-189 (Elsevier, United States, 2016). PMC5101015 * '''Methods for subtomogram averaging:''' * J.G. Galaz-Montoya, C.W. Hecksel, P.R. Baldwin, E. Wang, S.C. Weaver, M.F. Schmid, S.J. Ludtke & W. Chiu. (2016) Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography. J Struct Biol. 194, 383-394. PMC4846534 == Download EMAN2 == * [[EMAN2/Eman22Release|EMAN2.2 Release Notes]] * [[http://ncmi.bcm.tmc.edu/ncmi/software/software_details?selected_software=counter_222|Main EMAN2 Binary Download Page]] * [[https://github.com/cryoem/eman2|Source code from GitHub]] == Install EMAN2 == * [[EMAN2/Install|Installation Instructions and Tips]] (binary and source) * [[EMAN2/Remote|Strategies for using the GUI remotely (clusters or remote workstations)]] == Documentation == * Live Tutorials * Past video tutorials: [[https://www.youtube.com/c/SteveLudtke]] |
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| * [[EMAN2/Tutorials|EMAN2 Tutorials (START HERE!)]] * [[EMAN2/Programs|Individual Programs]] * [[EMAN2/Concepts|Concepts and Conventions in EMAN2]] |
* [[EMAN2/Tutorials|Tutorials (START HERE!)]] * [[EMAN2/DirectoryStructure|Folders and files in an EMAN2 Project]] * [[EMAN2/Concepts|File Formats, Symmetry, Box Size, ...]] * [[EMAN2/Programs|Individual Program Documentation]] |
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| * [[EMAN2/Eman1Transition|EMAN1 -> EMAN2 Transition Guide]] * [[EMAN2/Gpu|GPGPU Computing (use the graphics processor for image processing)]] |
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| * [[EMAN2/Obsolete|Obsolete Pages]] | |
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| * [[EMAN2/FAQ|FAQ - Ask your questions here]] | * [[http://blake.grid.bcm.edu/eman2/doxygen_html/classEMAN_1_1EMData.html|Direct link to docs for EMData (image) class]] * [[http://blake.grid.bcm.edu/eman2/doxygen_html/classEMAN_1_1Transform.html|Direct link to docs for Transform (orientation/Euler angle) class]] * [[EMAN2/GitTutorials|Transitioning from CVS to Git (under construction)]] * [[EMAN2/FAQ|FAQ]] - Please ask your questions in the Google Group, answers to common questions will be posted here as well as in the Group * Mailing list for EMAN2 discussions: * http://groups.google.com/group/eman2 * eman2@googlegroups.com (must join the group before you can post) * http://groups.google.com/group/eman2-developers (This is the group for discussions among developers, likely less interesting for users) |
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| ==== About EMAN2 ==== EMAN2 is the successor to [[EMAN1|EMAN1]]. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. Image processing in a suite like EMAN differs from consumer image processing packages like Photoshop in that pixels in images are represented as floating-point numbers rather than small (8-16 bit) integers. In addition, image compression is avoided entirely, and there is a focus on quantitative analysis rather than qualitative image display. |
== About EMAN2 == EMAN2 is the successor to [[EMAN1]]. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. Image processing in a suite like EMAN differs from consumer image processing packages like Photoshop in that pixels in images are represented as floating-point numbers rather than small (8-16 bit) integers. In addition, image compression is avoided entirely, and there is a focus on quantitative analysis rather than qualitative image display. |
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| EMAN2 is now a fully usable reconstruction package, including initial parallelism support. However, it was designed so both EMAN1 and EMAN2 can be installed in the same user account with no conflicts, in case you need some EMAN1 functionality. All EMAN2 programs, including GUI programs are written in the easy-to-learn Python scripting language. This permits knowledgable end-users to customize any of the code with unprecendented ease. If you aren't an advanced user, you can still make use of all of EMAN2's command-line programs, all of which start with 'e2'. Any programs in EMAN2 with an EMAN1 equivalent are likely substantially improved over their EMAN1 equivalents. For example e2pdb2mrc.py is ~10x faster than the EMAN1 pdb2mrc. | All EMAN2 programs, including GUI programs, are written in the easy-to-learn Python scripting language. This permits knowledgeable end-users to customize any of the code with unprecedented ease. If you aren't an advanced user, you can still make use of the integrated GUI and all of EMAN2's command-line programs. |
EMAN2.2
Most of the pages are editable by any user that has registered an account on the server. To prevent spam, you need to email sludtke@bcm.edu to get an account on the system if you wish to contribute changes. If you just wish to browse, you don't need an account.
EMAN2 is the successor to EMAN1. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. EMAN2 is capable of processing very large data sets (>100,000 particle) very efficiently (up to 20x faster than EMAN1).
Please also note that this is not the (related) EMEN2 electronic notebook, but is EMAN2, a scientific image processing suite.
Please Cite
EMAN is free software, supported by NIH Grants. It is critical that you cite EMAN2 when you use it in a publication in any significant way, to help us document usage when trying to renew our funding. Feel free to cite any of these:
Primary EMAN2 paper:
G. Tang, L. Peng, P.R. Baldwin, D.S. Mann, W. Jiang, I. Rees & S.J. Ludtke. (2007) EMAN2: an extensible image processing suite for electron microscopy. J Struct Biol. 157, 38-46. PMID: 16859925
EMAN2 high resolution refinement methods:
J.M. Bell, M. Chen, P.R. Baldwin & S.J. Ludtke. (2016) High Resolution Single Particle Refinement in EMAN2.1. Methods. 100, 25-34. PMC4848122
Methods for analysis of conformational and compositional variability:
- Ludtke, S. J. "Single-Particle Refinement and Variability Analysis in EMAN2.1." in Methods Enzymol 579159-189 (Elsevier, United States, 2016). PMC5101015
Methods for subtomogram averaging:
J.G. Galaz-Montoya, C.W. Hecksel, P.R. Baldwin, E. Wang, S.C. Weaver, M.F. Schmid, S.J. Ludtke & W. Chiu. (2016) Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography. J Struct Biol. 194, 383-394. PMC4846534
Download EMAN2
Install EMAN2
Installation Instructions and Tips (binary and source)
Strategies for using the GUI remotely (clusters or remote workstations)
Documentation
- Live Tutorials
Past video tutorials: https://www.youtube.com/c/SteveLudtke
- User Documentation
Advanced Users & Programmers
FAQ - Please ask your questions in the Google Group, answers to common questions will be posted here as well as in the Group
- Mailing list for EMAN2 discussions:
eman2@googlegroups.com (must join the group before you can post)
http://groups.google.com/group/eman2-developers (This is the group for discussions among developers, likely less interesting for users)
About EMAN2
EMAN2 is the successor to EMAN1. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. Image processing in a suite like EMAN differs from consumer image processing packages like Photoshop in that pixels in images are represented as floating-point numbers rather than small (8-16 bit) integers. In addition, image compression is avoided entirely, and there is a focus on quantitative analysis rather than qualitative image display.
All EMAN2 programs, including GUI programs, are written in the easy-to-learn Python scripting language. This permits knowledgeable end-users to customize any of the code with unprecedented ease. If you aren't an advanced user, you can still make use of the integrated GUI and all of EMAN2's command-line programs.
