Usage
msa <input footprint file> <output footprint file> <number
of components>
<input footprint file> | N (the number of original images) by Nfp (number of footprints used). |
<output footprint file> | N by Nfinal |
<# components> | Nfinal - number of footprint variables too keep in the output file |
Typical Usage
msa start.fp start.msa.fp 6
Description
This program will use principal component analysis to reduce the dimensionality
of a set of footprints. Depending on which
footprint program is used, each footprint will typically contain 10-100
components. msa reduces this dimensionality to a given value by finding
the most significant vectors in n-dimensional space and reprojecting the
footprints along them. The resulting footprint file should provide less
noisy representations of the original images.
msa is typically used just before running classes or classesrefs on the footprint files.
EMAN Copyright 1997-8 Steve Ludtke, Phil Baldwin