Reconstruction Flowchart

Note: This flowchart is designed to show you how EMAN processes data, not to teach you how to use it. If you'd like to learn how to do a reconstruction, follow the customized 4 step reconstruction process provided by eman

Click on a flowchart element for details.
 

Generate Initial Model

(particles with at least 3-fold rotational symmetry)

The process begins with boxed particlesThe particles with the best symmetry are selectedAlign and average these symmetric particlesOne euler angle still needs to be determinedUse the class averages to build a rough 3D model
 
 

Refine 3D model

Determine the CTF parameters for particles from each micrographStart with boxed particlesWe also have a preliminary 3D modelGenerate projections from 3d modelClassify particles by projection matchingUse class averages to build 3D modelAlign and average particles within each classClass averages are CTF corrected when CTF parameters are present
 

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